LIPO-STEM DUO

Adipose tissue MSCs microfragmentation and purification kit

 

LIPO-STEM DUO is a closed-circuit single-use kit for adipose tissue microfragmentation and purification without any centrifuge and with a minimal manipulation.

The filtering system consistently microfragments the adipose tissue while manteining its biological properties and maximizing the regenerative potential.

 

The entire processing phase of the liposuctioned tissue occurs inside the device thanks to continuous saline solution washing. This allows to reduce the cellular stress eliminating any traumatic action that may damage the extracellular matrix and its essential trophic and anti-inflammatory function.

The collection and processing bag is equipped with two filters:

  • the first filter microfragments the adipose tissue, while retaining the eventual fibrotic tissue
  • the second filter with denser mesh retains the reduced (microfragmented) adipose tissue that is washed with saline solution eliminating all the oily and blood residues which might cause inflammation of the treated tissues.

The final microfragmented and purified product is an autologous adipose tissue that keeps the biological properties of the original tissue intact and can easily be injected even through very thin needles.

KEY ADVANTAGES
 
  • No centrifugation required

  • Adipose tissue microfragmentation and purification

  • Preservation of extracellular matrix and stromal niche components

  • Total purification of blood and oily waste as potential cause of inflammation

  • Minimal manipulation thanks to a simple and reproducible technique in a single surgical time

  • Sampling and processing in a sterile closed-circuit environment

FIELDS OF APPLICATION
 
  • ORTHOPAEDICS

  • MAXILLOFACIAL SURGERY

  • SPINAL SURGERY

  • PAIN THERAPY

  • VULNOTHERAPY

  • PLASTIC AND RECONSTRUCTIVE SURGERY

  • GENERAL/UROGYNAECOLOGICAL SURGERY

HOW DOES IT WORK?

SURGICAL TECHNIQUE

1. Infiltrate the Klein solution in the selected harvest site using the 16 G Klein cannula

2. Harvest the adipose tissue from the anesthetized area using the 13 G lipoaspiration cannula

3. Connect a 2L saline bag to LIPO-STEM DUO “WASH IN” valve and fill the device.

4. Insert the harvested adipose tissue in the LIPO-STEM DUO “LIPO IN” valve.

5. The collection and processing bag is equipped with two filters: the first filter microfragments the adipose tissue, while retaining the eventual fibrotic tissue.

6. Support the purification of the adipose tissue using the processing spatula. This will gently microfragment and purify the adipose tissue.

 

7. The second filter with a denser mesh retains the microfragmented adipose tissue and discharges the pro-inflammatory contents and liquids in the waste bag.

8. The eventual fibrotic tissue is retained in the first filter, allowing to obtain a high qualitative adipose tissue.

9. Continue to gently move the processing spatula on the filter to facilitate the complete washing of the tissue.

9. Once the final product acquires a clear yellow color, stop the saline solution flow and gently remove the excess of water using the processing spatula.

 

10. With the help of the processing spatula retrieve the purified and microfragmented adipose tissue from the dedicated “LIPO OUT” valve and use as needed.

COMPONENTS

– LIPO-STEM DUO device

The device is supplied ready-to-use with a 400 ml capacity processing bag and a 2 lt waste collecting bag. The kit includes a processing spatula to facilitate the tissue purification.

The system has 3 valves:

       – WASH IN: to connect the saline solution

      – LIPO IN: to transfer the harvested adipose tissue into the device

      – LIPO OUT: to extract the microfragmented and purified adipose tissue.

IN VALVES

dettaglio valvole in

 

OUT VALVE

adipostem-det-down-trasp

– Filtering system

The sophisticated filtering system inside the processing and filtering bag is equipped with two filters:

  • the first filter microfragments the adipose tissue, while retaining the eventual fibrotic tissue
  • the second filter with denser mesh retains the reduced (microfragmented) adipose tissue that is washed with saline solution eliminating all the oily and blood residues which might cause inflammation of the treated tissues. 

– 16 G Klein solution cannula with 60 ml syringe

The kit includes:

  • two 60 ml syringes for Klein solution infiltration
  • one 16 G cannula with atraumatic tip and three side holes for Klein solution infiltration.
Klein Cannula 16G

16 G CANNULA TIP

– 13 G Atraumatic lipo-aspiration cannula with 60 ml Vaclok syringe

The kit includes:

  • two 60 ml Vaclok syringes for lipoaspiration
  • one 13 G lipoaspiration cannula with atraumatic tip and three ultrasharp side holes that allow to harvest a high qualitative and vital adipose cluster.
Lipo Aspiration Cannula 13G
16G_V6A1948_black-hole_dettaglio_9

13 G CANNULA TIP

VIDEO TUTORIAL

RELATED PRODUCTs

LIPO-STEM MONO

Adipose tissue MSCs purification kit

 

MARROW-STEM

Bone marrow MSCs aspiration kit

 

OSTEOPLASTY

Metaphyseal fracture balloon augmentation

ORTHOPLASTY

Subchondral bone plasty